Off-the-shelf proximity biotinylation for interaction proteomics

Proximity biotinylation workflows typically require CRISPR-based genetic manipulation of target cells. To overcome this bottleneck, we fused the TurboID proximity biotinylation enzyme to Protein A. Upon target cell permeabilization, the ProtA-Turbo enzyme can be targeted to proteins or post-translat...

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Detalles Bibliográficos
Autores principales: Santos-Barriopedro, Irene, van Mierlo, Guido, Vermeulen, Michiel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8373943/
https://www.ncbi.nlm.nih.gov/pubmed/34408139
http://dx.doi.org/10.1038/s41467-021-25338-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8373943/
https://www.ncbi.nlm.nih.gov/pubmed/34408139
http://dx.doi.org/10.1038/s41467-021-25338-4

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