Cargando…

Off-the-shelf proximity biotinylation for interaction proteomics

Proximity biotinylation workflows typically require CRISPR-based genetic manipulation of target cells. To overcome this bottleneck, we fused the TurboID proximity biotinylation enzyme to Protein A. Upon target cell permeabilization, the ProtA-Turbo enzyme can be targeted to proteins or post-translat...

Descripción completa

Detalles Bibliográficos
Autores principales:	Santos-Barriopedro, Irene, van Mierlo, Guido, Vermeulen, Michiel
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8373943/ https://www.ncbi.nlm.nih.gov/pubmed/34408139 http://dx.doi.org/10.1038/s41467-021-25338-4

Ejemplares similares

Chromatin Proteomics to Study Epigenetics — Challenges and Opportunities
por: van Mierlo, Guido, et al.
Publicado: (2021)

Mitochondrially targeted proximity biotinylation and proteomic analysis in Plasmodium falciparum
por: Lamb, Ian M., et al.
Publicado: (2022)

Proximity-Dependent Biotinylation Approaches to Explore the Dynamic Compartmentalized Proteome
por: Dionne, Ugo, et al.
Publicado: (2022)

Proteomic analysis of HIV-1 Gag interacting partners using proximity-dependent biotinylation
por: Le Sage, Valerie, et al.
Publicado: (2015)

Screening of Proximal and Interacting Proteins in Rice Protoplasts by Proximity-Dependent Biotinylation
por: Lin, Qiupeng, et al.
Publicado: (2017)

DNA Modifications Enabling Proximity Biotinylation
por: Wilbanks, Brandon, et al.
Publicado: (2023)

Proximity Utilizing Biotinylation of Nuclear Proteins in vivo
por: Kulyyassov, Arman, et al.
Publicado: (2015)

Identification of Interactions in the NMD Complex Using Proximity-Dependent Biotinylation (BioID)
por: Schweingruber, Christoph, et al.
Publicado: (2016)

Autophagosome content profiling using proximity biotinylation proteomics coupled to protease digestion in mammalian cells
por: Zellner, Susanne, et al.
Publicado: (2021)

Proximity-dependent biotinylation technologies for mapping RNA-protein interactions in live cells
por: Giambruno, Roberto, et al.
Publicado: (2022)

In vivo discovery of RNA proximal proteins via proximity-dependent biotinylation
por: Lin, Xianzhi, et al.
Publicado: (2021)

Biotinylation by antibody recognition - A method for proximity labeling
por: Bar, Daniel Z, et al.
Publicado: (2017)

Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation
por: Hung, Victoria, et al.
Publicado: (2017)

AirID, a novel proximity biotinylation enzyme, for analysis of protein–protein interactions
por: Kido, Kohki, et al.
Publicado: (2020)

Insight into the Interactome of Intramitochondrial PKA Using Biotinylation-Proximity Labeling
por: Ould Amer, Yasmine, et al.
Publicado: (2020)

Proximity-dependent biotinylation and identification of flagellar proteins in Trypanosoma cruzi
por: Won, Madalyn M., et al.
Publicado: (2023)

Proximity-Dependent Biotinylation and Identification of Flagellar Proteins in Trypanosoma cruzi
por: Won, Madalyn M., et al.
Publicado: (2023)

A Toolbox for Efficient Proximity-Dependent Biotinylation in Zebrafish Embryos
por: Rosenthal, Shimon M., et al.
Publicado: (2021)

In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation
por: Gillingham, Alison K, et al.
Publicado: (2019)

Correction: Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation
por: Hung, Victoria, et al.
Publicado: (2019)

Carcinogens off the Shelf?
por: Stewart, Bernard W
Publicado: (2018)

Comparative Application of BioID and TurboID for Protein-Proximity Biotinylation
por: May, Danielle G., et al.
Publicado: (2020)

Mapping axon initial segment structure and function by multiplexed proximity biotinylation
por: Hamdan, Hamdan, et al.
Publicado: (2020)

Topological features of integrin adhesion complexes revealed by multiplexed proximity biotinylation
por: Chastney, Megan R., et al.
Publicado: (2020)

Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells
por: Chen, Rui, et al.
Publicado: (2022)

In vivo Biotinylation Based Method for the Study of Protein-Protein Proximity in Eukaryotic Cells
por: Kulyyassov, Arman, et al.
Publicado: (2014)

Generation of Peptides for Highly Efficient Proximity Utilizing Site-Specific Biotinylation in Cells
por: Kulyyassov, Arman, et al.
Publicado: (2022)

Off the Shelf Fouling Management
por: Rittschof, Daniel
Publicado: (2017)

Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis
por: Kiss, Robert S., et al.
Publicado: (2023)

Multiplex Detection of Homo- and Heterodimerization of G Protein-Coupled Receptors by Proximity Biotinylation
por: Steel, Elisabeth, et al.
Publicado: (2014)

Proximity-dependent biotinylation screening identifies NbHYPK as a novel interacting partner of ATG8 in plants
por: Macharia, Mercy W., et al.
Publicado: (2019)

A multi-layered structure of the interphase chromocenter revealed by proximity-based biotinylation
por: Kochanova, Natalia Y, et al.
Publicado: (2020)

Use of Biotinylated Ubiquitin for Analysis of Rat Brain Mitochondrial Proteome and Interactome
por: Buneeva, Olga A., et al.
Publicado: (2012)

A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues
por: Yamanaka, Satoshi, et al.
Publicado: (2022)

Proximity biotinylation reveals novel secreted dense granule proteins of Toxoplasma gondii bradyzoites
por: Nadipuram, Santhosh Mukund, et al.
Publicado: (2020)

Tag Thy Neighbour: Nanometre-Scale Insights Into Kinetoplastid Parasites With Proximity Dependent Biotinylation
por: Geoghegan, Vincent, et al.
Publicado: (2022)

Expressing the human proteome for affinity proteomics: optimising expression of soluble protein domains and in vivo biotinylation
por: Keates, Tracy, et al.
Publicado: (2012)

Engineering of ultraID, a compact and hyperactive enzyme for proximity-dependent biotinylation in living cells
por: Kubitz, Lea, et al.
Publicado: (2022)

Calibration of Off-the-Shelf Anisotropic Magnetoresistance Magnetometers
por: Schulz, Leonard, et al.
Publicado: (2019)

PUB-NChIP—“in vivo biotinylation” approach to study chromatin in proximity to a protein of interest
por: Shoaib, Muhammad, et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_l7nnrfsumjlc5bvr6iok3r0ukb