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A real-time (31)P-NMR-based approach for the assessment of glycerol kinase catalyzed monophosphorylations

Phosphorous-NMR is scarcely employed to evaluate enzyme kinetics of kinase driven monophosphorylations, despite of being a powerful and reliable tool to undoubtedly detect the actual phosphoryl transfer to the targeted substrate. Another advantage is that an external supplementation source of the NM...

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Detalles Bibliográficos
Autores principales: Escobedo-Hinojosa, Wendy, Wissner, Julian L., Hauer, Bernhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374273/
https://www.ncbi.nlm.nih.gov/pubmed/34434805
http://dx.doi.org/10.1016/j.mex.2021.101285