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A real-time (31)P-NMR-based approach for the assessment of glycerol kinase catalyzed monophosphorylations
Phosphorous-NMR is scarcely employed to evaluate enzyme kinetics of kinase driven monophosphorylations, despite of being a powerful and reliable tool to undoubtedly detect the actual phosphoryl transfer to the targeted substrate. Another advantage is that an external supplementation source of the NM...
Autores principales: | Escobedo-Hinojosa, Wendy, Wissner, Julian L., Hauer, Bernhard |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374273/ https://www.ncbi.nlm.nih.gov/pubmed/34434805 http://dx.doi.org/10.1016/j.mex.2021.101285 |
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