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Two plasmid modules for introducing the auxin-inducible degron into the fission yeast Schizosaccharomyces pombe by PCR-based gene targeting

Targeted protein degradation is a powerful approach to study and inhibit protein function in vivo. Introduction of the auxin-inducible degron (AID) system to the fission yeast Schizosaccharomyces pombe was previously reported, but, to the best of our knowledge, no plasmid for constructing AID-tagged...

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Detalles Bibliográficos
Autores principales: Song, Xiuyi, Xu, Ruoming, Sugiyama, Tomoyasu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374545/
https://www.ncbi.nlm.nih.gov/pubmed/34423281
http://dx.doi.org/10.17912/micropub.biology.000442