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A method for characterizing the thermal stability and antimicrobial binding to Lipopolysaccharides of Gram-negative isogenic mutant strains

Isothermal Titration Calorimetry (ITC) is widely employed to assess antimicrobial affinity for lipopolysaccharide (LPS); nevertheless, experiments are usually limited to commercially available-LPS chemotypes. Herein we show a method that uses Differential Scanning Calorimetry (DSC) to characterize h...

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Detalles Bibliográficos
Autores principales: Navarro, Belén, Alarcón, Mackarenna, Osees, Maricarmen, Gómez-Alvear, Felipe, Sepúlveda, Romina V., Huerta, Jaime, Opazo, María Cecilia, Aguayo, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374694/
https://www.ncbi.nlm.nih.gov/pubmed/34434873
http://dx.doi.org/10.1016/j.mex.2021.101474