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Regulated delivery controls Drosophila Hedgehog, Wingless, and Decapentaplegic signaling

Morphogen signaling proteins disperse across tissues to activate signal transduction in target cells. We investigated dispersion of Hedgehog (Hh), Wnt homolog Wingless (Wg), and Bone morphogenic protein homolog Decapentaplegic (Dpp) in the Drosophila wing imaginal disc. We discovered that delivery o...

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Autores principales: Hatori, Ryo, Wood, Brent M, Oliveira Barbosa, Guilherme, Kornberg, Thomas B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376250/
https://www.ncbi.nlm.nih.gov/pubmed/34292155
http://dx.doi.org/10.7554/eLife.71744
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author Hatori, Ryo
Wood, Brent M
Oliveira Barbosa, Guilherme
Kornberg, Thomas B
author_facet Hatori, Ryo
Wood, Brent M
Oliveira Barbosa, Guilherme
Kornberg, Thomas B
author_sort Hatori, Ryo
collection PubMed
description Morphogen signaling proteins disperse across tissues to activate signal transduction in target cells. We investigated dispersion of Hedgehog (Hh), Wnt homolog Wingless (Wg), and Bone morphogenic protein homolog Decapentaplegic (Dpp) in the Drosophila wing imaginal disc. We discovered that delivery of Hh, Wg, and Dpp to their respective targets is regulated. We found that <5% of Hh and <25% of Wg are taken up by disc cells and activate signaling. The amount of morphogen that is taken up and initiates signaling did not change when the level of morphogen expression was varied between 50 and 200% (Hh) or 50 and 350% (Wg). Similar properties were observed for Dpp. We analyzed an area of 150 μm×150 μm that includes Hh-responding cells of the disc as well as overlying tracheal cells and myoblasts that are also activated by disc-produced Hh. We found that the extent of signaling in the disc was unaffected by the presence or absence of the tracheal and myoblast cells, suggesting that the mechanism that disperses Hh specifies its destinations to particular cells, and that target cells do not take up Hh from a common pool.
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spelling pubmed-83762502021-08-20 Regulated delivery controls Drosophila Hedgehog, Wingless, and Decapentaplegic signaling Hatori, Ryo Wood, Brent M Oliveira Barbosa, Guilherme Kornberg, Thomas B eLife Developmental Biology Morphogen signaling proteins disperse across tissues to activate signal transduction in target cells. We investigated dispersion of Hedgehog (Hh), Wnt homolog Wingless (Wg), and Bone morphogenic protein homolog Decapentaplegic (Dpp) in the Drosophila wing imaginal disc. We discovered that delivery of Hh, Wg, and Dpp to their respective targets is regulated. We found that <5% of Hh and <25% of Wg are taken up by disc cells and activate signaling. The amount of morphogen that is taken up and initiates signaling did not change when the level of morphogen expression was varied between 50 and 200% (Hh) or 50 and 350% (Wg). Similar properties were observed for Dpp. We analyzed an area of 150 μm×150 μm that includes Hh-responding cells of the disc as well as overlying tracheal cells and myoblasts that are also activated by disc-produced Hh. We found that the extent of signaling in the disc was unaffected by the presence or absence of the tracheal and myoblast cells, suggesting that the mechanism that disperses Hh specifies its destinations to particular cells, and that target cells do not take up Hh from a common pool. eLife Sciences Publications, Ltd 2021-07-22 /pmc/articles/PMC8376250/ /pubmed/34292155 http://dx.doi.org/10.7554/eLife.71744 Text en © 2021, Hatori et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology
Hatori, Ryo
Wood, Brent M
Oliveira Barbosa, Guilherme
Kornberg, Thomas B
Regulated delivery controls Drosophila Hedgehog, Wingless, and Decapentaplegic signaling
title Regulated delivery controls Drosophila Hedgehog, Wingless, and Decapentaplegic signaling
title_full Regulated delivery controls Drosophila Hedgehog, Wingless, and Decapentaplegic signaling
title_fullStr Regulated delivery controls Drosophila Hedgehog, Wingless, and Decapentaplegic signaling
title_full_unstemmed Regulated delivery controls Drosophila Hedgehog, Wingless, and Decapentaplegic signaling
title_short Regulated delivery controls Drosophila Hedgehog, Wingless, and Decapentaplegic signaling
title_sort regulated delivery controls drosophila hedgehog, wingless, and decapentaplegic signaling
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376250/
https://www.ncbi.nlm.nih.gov/pubmed/34292155
http://dx.doi.org/10.7554/eLife.71744
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