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Heat treatment of thioredoxin fusions increases the purity of α‐helical transmembrane protein constructs

Membrane proteins play key roles in cellular signaling and transport, represent the majority of drug targets, and are implicated in many diseases. Their relevance renders them important subjects for structural, biophysical, and functional investigations. However, obtaining membrane proteins in high...

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Detalles Bibliográficos
Autores principales: Schenkel, Mathias, Treff, Antoine, Deber, Charles M., Krainer, Georg, Schlierf, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376418/
https://www.ncbi.nlm.nih.gov/pubmed/34191368
http://dx.doi.org/10.1002/pro.4150