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Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity
CRISPR-Cas9 is a powerful DNA editing tool. A gRNA directs Cas9 to cleave any DNA sequence with a PAM. However, some gRNA sequences mediate cleavage at higher efficiencies than others. To understand this, numerous studies have screened large gRNA libraries and developed algorithms to predict gRNA se...
| Autors principals: | , |
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| Format: | Online Article Text |
| Idioma: | English |
| Publicat: |
Nature Publishing Group UK
2021
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| Matèries: | |
| Accés en línia: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377084/ https://www.ncbi.nlm.nih.gov/pubmed/34413309 http://dx.doi.org/10.1038/s41467-021-25339-3 |