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Optimization of mito-roGFP protocol to measure mitochondrial oxidative status in human coronary artery endothelial cells

Reactive oxygen species (ROS) are implicated in endothelial dysfunction and cardiovascular disease. Endothelial cells (ECs) produce most ATP through glycolysis rather than oxidative phosphorylation; thus mitochondrial ROS production is lower than in other cell types. This makes quantification of cha...

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Detalles Bibliográficos
Autores principales: Teixeira, Rayane Brinck, Karbasiafshar, Catherine, Sabra, Mohamed, Abid, M. Ruhul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377591/
https://www.ncbi.nlm.nih.gov/pubmed/34458871
http://dx.doi.org/10.1016/j.xpro.2021.100753