A modified CUT&RUN protocol and analysis pipeline to identify transcription factor binding sites in human cell lines

CUT&RUN is a recently developed in situ chromatin profiling technique that enables high-resolution chromatin mapping and probing. Herein, we describe our adapted CUT&RUN protocol for transcription factors (TFs). Our protocol outlines all necessary steps for TF profiling including the procedu...

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Detalles Bibliográficos
Autores principales: Kong, Nikki Ruoxi, Chai, Li, Tenen, Daniel Geoffrey, Bassal, Mahmoud Adel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379522/
https://www.ncbi.nlm.nih.gov/pubmed/34458869
http://dx.doi.org/10.1016/j.xpro.2021.100750

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379522/
https://www.ncbi.nlm.nih.gov/pubmed/34458869
http://dx.doi.org/10.1016/j.xpro.2021.100750

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