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A modified CUT&RUN protocol and analysis pipeline to identify transcription factor binding sites in human cell lines
CUT&RUN is a recently developed in situ chromatin profiling technique that enables high-resolution chromatin mapping and probing. Herein, we describe our adapted CUT&RUN protocol for transcription factors (TFs). Our protocol outlines all necessary steps for TF profiling including the procedu...
Autores principales: | Kong, Nikki Ruoxi, Chai, Li, Tenen, Daniel Geoffrey, Bassal, Mahmoud Adel |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379522/ https://www.ncbi.nlm.nih.gov/pubmed/34458869 http://dx.doi.org/10.1016/j.xpro.2021.100750 |
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