Wobble Editing of Cre-box by Unspecific CRISPR/Cas9 Causes CCR Release and Phenotypic Changes in Bacillus pumilus

CRISPR-associated Cas9 endonuclease (CRISPR/Cas9) systems are widely used to introduce precise mutations, such as knocking in/out at targeted genomic sites. Herein, we successfully disrupted the transcription of multiple genes in Bacillus pumilus LG3145 using a series of unspecific guide RNAs (gRNAs...

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Detalles Bibliográficos
Autores principales: Wang, Yingxiang, Cao, Linfeng, Bi, Meiying, Wang, Sicheng, Chen, Meiting, Chen, Xingyu, Ying, Ming, Huang, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8381255/
https://www.ncbi.nlm.nih.gov/pubmed/34434920
http://dx.doi.org/10.3389/fchem.2021.717609

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8381255/
https://www.ncbi.nlm.nih.gov/pubmed/34434920
http://dx.doi.org/10.3389/fchem.2021.717609

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