Simultaneous knockout of multiple LHCF genes using single sgRNAs and engineering of a high‐fidelity Cas9 for precise genome editing in marine algae

The CRISPR/Cas9 system is an RNA‐guided sequence‐specific genome editing tool, which has been adopted for single or multiple gene editing in a wide range of organisms. When working with gene families with functional redundancy, knocking out multiple genes within the same family may be required to ge...

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Detalles Bibliográficos
Autores principales: Sharma, Amit K., Nymark, Marianne, Flo, Snorre, Sparstad, Torfinn, Bones, Atle M., Winge, Per
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384595/
https://www.ncbi.nlm.nih.gov/pubmed/33759354
http://dx.doi.org/10.1111/pbi.13582

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384595/
https://www.ncbi.nlm.nih.gov/pubmed/33759354
http://dx.doi.org/10.1111/pbi.13582

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