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Simultaneous knockout of multiple LHCF genes using single sgRNAs and engineering of a high‐fidelity Cas9 for precise genome editing in marine algae
The CRISPR/Cas9 system is an RNA‐guided sequence‐specific genome editing tool, which has been adopted for single or multiple gene editing in a wide range of organisms. When working with gene families with functional redundancy, knocking out multiple genes within the same family may be required to ge...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384595/ https://www.ncbi.nlm.nih.gov/pubmed/33759354 http://dx.doi.org/10.1111/pbi.13582 |