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Utilizing thermal proteome profiling to identify the molecular targets of anti-leishmanial compounds

Here, we detail our optimized protocol for the identification of drug targets in Leishmania donovani using thermal proteome profiling. This approach is based on the principle that binding of a drug to its protein target can significantly alter the thermal stability of that protein. By monitoring cha...

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Detalles Bibliográficos
Autores principales: Corpas-Lopez, Victoriano, Wyllie, Susan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384900/
https://www.ncbi.nlm.nih.gov/pubmed/34467225
http://dx.doi.org/10.1016/j.xpro.2021.100704
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author Corpas-Lopez, Victoriano
Wyllie, Susan
author_facet Corpas-Lopez, Victoriano
Wyllie, Susan
author_sort Corpas-Lopez, Victoriano
collection PubMed
description Here, we detail our optimized protocol for the identification of drug targets in Leishmania donovani using thermal proteome profiling. This approach is based on the principle that binding of a drug to its protein target can significantly alter the thermal stability of that protein. By monitoring changes in the thermal stability of proteins within drug-treated and untreated cell lysates, using mass spectrometry combined with tandem mass tag labeling, putative targets of the drug can be identified in an unbiased manner. For further details on the use and application of this protocol, please refer to Paradela et al. (2021).
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spelling pubmed-83849002021-08-30 Utilizing thermal proteome profiling to identify the molecular targets of anti-leishmanial compounds Corpas-Lopez, Victoriano Wyllie, Susan STAR Protoc Protocol Here, we detail our optimized protocol for the identification of drug targets in Leishmania donovani using thermal proteome profiling. This approach is based on the principle that binding of a drug to its protein target can significantly alter the thermal stability of that protein. By monitoring changes in the thermal stability of proteins within drug-treated and untreated cell lysates, using mass spectrometry combined with tandem mass tag labeling, putative targets of the drug can be identified in an unbiased manner. For further details on the use and application of this protocol, please refer to Paradela et al. (2021). Elsevier 2021-08-18 /pmc/articles/PMC8384900/ /pubmed/34467225 http://dx.doi.org/10.1016/j.xpro.2021.100704 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Corpas-Lopez, Victoriano
Wyllie, Susan
Utilizing thermal proteome profiling to identify the molecular targets of anti-leishmanial compounds
title Utilizing thermal proteome profiling to identify the molecular targets of anti-leishmanial compounds
title_full Utilizing thermal proteome profiling to identify the molecular targets of anti-leishmanial compounds
title_fullStr Utilizing thermal proteome profiling to identify the molecular targets of anti-leishmanial compounds
title_full_unstemmed Utilizing thermal proteome profiling to identify the molecular targets of anti-leishmanial compounds
title_short Utilizing thermal proteome profiling to identify the molecular targets of anti-leishmanial compounds
title_sort utilizing thermal proteome profiling to identify the molecular targets of anti-leishmanial compounds
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384900/
https://www.ncbi.nlm.nih.gov/pubmed/34467225
http://dx.doi.org/10.1016/j.xpro.2021.100704
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