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A modified density gradient proteomic-based method to analyze endolysosomal proteins in cardiac tissue

The importance of lysosomes in cardiac physiology and pathology is well established, and evidence for roles in calcium signaling is emerging. We describe a label-free proteomics method suitable for small cardiac tissue biopsies based on density-separated fractionation, which allows study of endolyso...

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Detalles Bibliográficos
Autores principales: Ayagama, Thamali, Bose, Samuel J., Capel, Rebecca A., Priestman, David A., Berridge, Georgina, Fischer, Roman, Galione, Antony, Platt, Frances M., Kramer, Holger, Burton, Rebecca A.B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384914/
https://www.ncbi.nlm.nih.gov/pubmed/34466782
http://dx.doi.org/10.1016/j.isci.2021.102949