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The structure of a dimeric form of SARS-CoV-2 polymerase

The coronavirus SARS-CoV-2 uses an RNA-dependent RNA polymerase (RdRp) to replicate and transcribe its genome. Previous structures of the RdRp revealed a monomeric enzyme composed of the catalytic subunit nsp12, two copies of subunit nsp8, and one copy of subunit nsp7. Here we report an alternative,...

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Autores principales: Jochheim, Florian A., Tegunov, Dimitry, Hillen, Hauke S., Schmitzová, Jana, Kokic, Goran, Dienemann, Christian, Cramer, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385044/
https://www.ncbi.nlm.nih.gov/pubmed/34429502
http://dx.doi.org/10.1038/s42003-021-02529-9
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author Jochheim, Florian A.
Tegunov, Dimitry
Hillen, Hauke S.
Schmitzová, Jana
Kokic, Goran
Dienemann, Christian
Cramer, Patrick
author_facet Jochheim, Florian A.
Tegunov, Dimitry
Hillen, Hauke S.
Schmitzová, Jana
Kokic, Goran
Dienemann, Christian
Cramer, Patrick
author_sort Jochheim, Florian A.
collection PubMed
description The coronavirus SARS-CoV-2 uses an RNA-dependent RNA polymerase (RdRp) to replicate and transcribe its genome. Previous structures of the RdRp revealed a monomeric enzyme composed of the catalytic subunit nsp12, two copies of subunit nsp8, and one copy of subunit nsp7. Here we report an alternative, dimeric form of the enzyme and resolve its structure at 5.5 Å resolution. In this structure, the two RdRps contain only one copy of nsp8 each and dimerize via their nsp7 subunits to adopt an antiparallel arrangement. We speculate that the RdRp dimer facilitates template switching during production of sub-genomic RNAs.
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spelling pubmed-83850442021-09-22 The structure of a dimeric form of SARS-CoV-2 polymerase Jochheim, Florian A. Tegunov, Dimitry Hillen, Hauke S. Schmitzová, Jana Kokic, Goran Dienemann, Christian Cramer, Patrick Commun Biol Article The coronavirus SARS-CoV-2 uses an RNA-dependent RNA polymerase (RdRp) to replicate and transcribe its genome. Previous structures of the RdRp revealed a monomeric enzyme composed of the catalytic subunit nsp12, two copies of subunit nsp8, and one copy of subunit nsp7. Here we report an alternative, dimeric form of the enzyme and resolve its structure at 5.5 Å resolution. In this structure, the two RdRps contain only one copy of nsp8 each and dimerize via their nsp7 subunits to adopt an antiparallel arrangement. We speculate that the RdRp dimer facilitates template switching during production of sub-genomic RNAs. Nature Publishing Group UK 2021-08-24 /pmc/articles/PMC8385044/ /pubmed/34429502 http://dx.doi.org/10.1038/s42003-021-02529-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Jochheim, Florian A.
Tegunov, Dimitry
Hillen, Hauke S.
Schmitzová, Jana
Kokic, Goran
Dienemann, Christian
Cramer, Patrick
The structure of a dimeric form of SARS-CoV-2 polymerase
title The structure of a dimeric form of SARS-CoV-2 polymerase
title_full The structure of a dimeric form of SARS-CoV-2 polymerase
title_fullStr The structure of a dimeric form of SARS-CoV-2 polymerase
title_full_unstemmed The structure of a dimeric form of SARS-CoV-2 polymerase
title_short The structure of a dimeric form of SARS-CoV-2 polymerase
title_sort structure of a dimeric form of sars-cov-2 polymerase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385044/
https://www.ncbi.nlm.nih.gov/pubmed/34429502
http://dx.doi.org/10.1038/s42003-021-02529-9
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