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Host-associated microbe PCR (hamPCR) enables convenient measurement of both microbial load and community composition

The ratio of microbial population size relative to the amount of host tissue, or ‘microbial load’, is a fundamental metric of colonization and infection, but it cannot be directly deduced from microbial amplicon data such as 16S rRNA gene counts. Because existing methods to determine load, such as s...

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Detalles Bibliográficos
Autores principales: Lundberg, Derek S, Pramoj Na Ayutthaya, Pratchaya, Strauß, Annett, Shirsekar, Gautam, Lo, Wen-Sui, Lahaye, Thomas, Weigel, Detlef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387020/
https://www.ncbi.nlm.nih.gov/pubmed/34292157
http://dx.doi.org/10.7554/eLife.66186