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Protocol for intracellular and extracellular metabolite detection in human embryonic stem cells

Metabolic homeostasis is critical for cell pluripotency and differentiation in human embryonic stem cells (hESCs). It has been reported that metabolic changes specifically regulate cellular signaling during hESC differentiation. This protocol describes procedures for both cell culture and detection...

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Detalles Bibliográficos
Autores principales: Xu, Faxiang, Song, Chengcheng, Liu, Weiwei, Chen, Guokai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387572/
https://www.ncbi.nlm.nih.gov/pubmed/34467226
http://dx.doi.org/10.1016/j.xpro.2021.100740