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Protocol for intracellular and extracellular metabolite detection in human embryonic stem cells
Metabolic homeostasis is critical for cell pluripotency and differentiation in human embryonic stem cells (hESCs). It has been reported that metabolic changes specifically regulate cellular signaling during hESC differentiation. This protocol describes procedures for both cell culture and detection...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387572/ https://www.ncbi.nlm.nih.gov/pubmed/34467226 http://dx.doi.org/10.1016/j.xpro.2021.100740 |