Cargando…

Protocol for intracellular and extracellular metabolite detection in human embryonic stem cells

Metabolic homeostasis is critical for cell pluripotency and differentiation in human embryonic stem cells (hESCs). It has been reported that metabolic changes specifically regulate cellular signaling during hESC differentiation. This protocol describes procedures for both cell culture and detection...

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Faxiang, Song, Chengcheng, Liu, Weiwei, Chen, Guokai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387572/
https://www.ncbi.nlm.nih.gov/pubmed/34467226
http://dx.doi.org/10.1016/j.xpro.2021.100740
_version_ 1783742469596250112
author Xu, Faxiang
Song, Chengcheng
Liu, Weiwei
Chen, Guokai
author_facet Xu, Faxiang
Song, Chengcheng
Liu, Weiwei
Chen, Guokai
author_sort Xu, Faxiang
collection PubMed
description Metabolic homeostasis is critical for cell pluripotency and differentiation in human embryonic stem cells (hESCs). It has been reported that metabolic changes specifically regulate cellular signaling during hESC differentiation. This protocol describes procedures for both cell culture and detection of intracellular and extracellular metabolites in hESCs by liquid chromatography-mass spectrometry. Metabolites in glycolysis, citric acid cycle, pentose phosphate pathway, and other metabolic processes can be detected using this approach. For complete details on the use and execution of this protocol, please refer to Song et al., (2019), Yang et al., (2019), Meng et al., (2018), and Chen et al., (2011b).
format Online
Article
Text
id pubmed-8387572
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-83875722021-08-30 Protocol for intracellular and extracellular metabolite detection in human embryonic stem cells Xu, Faxiang Song, Chengcheng Liu, Weiwei Chen, Guokai STAR Protoc Protocol Metabolic homeostasis is critical for cell pluripotency and differentiation in human embryonic stem cells (hESCs). It has been reported that metabolic changes specifically regulate cellular signaling during hESC differentiation. This protocol describes procedures for both cell culture and detection of intracellular and extracellular metabolites in hESCs by liquid chromatography-mass spectrometry. Metabolites in glycolysis, citric acid cycle, pentose phosphate pathway, and other metabolic processes can be detected using this approach. For complete details on the use and execution of this protocol, please refer to Song et al., (2019), Yang et al., (2019), Meng et al., (2018), and Chen et al., (2011b). Elsevier 2021-08-12 /pmc/articles/PMC8387572/ /pubmed/34467226 http://dx.doi.org/10.1016/j.xpro.2021.100740 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Xu, Faxiang
Song, Chengcheng
Liu, Weiwei
Chen, Guokai
Protocol for intracellular and extracellular metabolite detection in human embryonic stem cells
title Protocol for intracellular and extracellular metabolite detection in human embryonic stem cells
title_full Protocol for intracellular and extracellular metabolite detection in human embryonic stem cells
title_fullStr Protocol for intracellular and extracellular metabolite detection in human embryonic stem cells
title_full_unstemmed Protocol for intracellular and extracellular metabolite detection in human embryonic stem cells
title_short Protocol for intracellular and extracellular metabolite detection in human embryonic stem cells
title_sort protocol for intracellular and extracellular metabolite detection in human embryonic stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387572/
https://www.ncbi.nlm.nih.gov/pubmed/34467226
http://dx.doi.org/10.1016/j.xpro.2021.100740
work_keys_str_mv AT xufaxiang protocolforintracellularandextracellularmetabolitedetectioninhumanembryonicstemcells
AT songchengcheng protocolforintracellularandextracellularmetabolitedetectioninhumanembryonicstemcells
AT liuweiwei protocolforintracellularandextracellularmetabolitedetectioninhumanembryonicstemcells
AT chenguokai protocolforintracellularandextracellularmetabolitedetectioninhumanembryonicstemcells