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Integrated Phenotypic–Genotypic Analysis of Latilactobacillus sakei from Different Niches

Increasing attention has been paid to the potential probiotic effects of Latilactobacillus sakei. To explore the genetic diversity of L. sakei, 14 strains isolated from different niches (feces, fermented kimchi, and meat products) and 54 published strains were compared and analyzed. The results show...

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Detalles Bibliográficos
Autores principales: Chen, Ying, Li, Nan, Zhao, Shenxi, Zhang, Chuan, Qiao, Nanzhen, Duan, Hui, Xiao, Yue, Yan, Bowen, Zhao, Jianxin, Tian, Fengwei, Zhai, Qixiao, Yu, Leilei, Chen, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393274/
https://www.ncbi.nlm.nih.gov/pubmed/34441495
http://dx.doi.org/10.3390/foods10081717
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author Chen, Ying
Li, Nan
Zhao, Shenxi
Zhang, Chuan
Qiao, Nanzhen
Duan, Hui
Xiao, Yue
Yan, Bowen
Zhao, Jianxin
Tian, Fengwei
Zhai, Qixiao
Yu, Leilei
Chen, Wei
author_facet Chen, Ying
Li, Nan
Zhao, Shenxi
Zhang, Chuan
Qiao, Nanzhen
Duan, Hui
Xiao, Yue
Yan, Bowen
Zhao, Jianxin
Tian, Fengwei
Zhai, Qixiao
Yu, Leilei
Chen, Wei
author_sort Chen, Ying
collection PubMed
description Increasing attention has been paid to the potential probiotic effects of Latilactobacillus sakei. To explore the genetic diversity of L. sakei, 14 strains isolated from different niches (feces, fermented kimchi, and meat products) and 54 published strains were compared and analyzed. The results showed that the average genome size and GC content of L. sakei were 1.98 Mb and 41.22%, respectively. Its core genome mainly encodes translation and transcription, amino acid synthesis, glucose metabolism, and defense functions. L. sakei has open pan-genomic characteristics, and its pan-gene curve shows an upward trend. The genetic diversity of L. sakei is mainly reflected in carbohydrate utilization, antibiotic tolerance, and immune/competition-related factors, such as clustering regular interval short palindromic repeat sequence (CRISPR)–Cas. The CRISPR system is mainly IIA type, and a few are IIC types. This work provides a basis for the study of this species.
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spelling pubmed-83932742021-08-28 Integrated Phenotypic–Genotypic Analysis of Latilactobacillus sakei from Different Niches Chen, Ying Li, Nan Zhao, Shenxi Zhang, Chuan Qiao, Nanzhen Duan, Hui Xiao, Yue Yan, Bowen Zhao, Jianxin Tian, Fengwei Zhai, Qixiao Yu, Leilei Chen, Wei Foods Article Increasing attention has been paid to the potential probiotic effects of Latilactobacillus sakei. To explore the genetic diversity of L. sakei, 14 strains isolated from different niches (feces, fermented kimchi, and meat products) and 54 published strains were compared and analyzed. The results showed that the average genome size and GC content of L. sakei were 1.98 Mb and 41.22%, respectively. Its core genome mainly encodes translation and transcription, amino acid synthesis, glucose metabolism, and defense functions. L. sakei has open pan-genomic characteristics, and its pan-gene curve shows an upward trend. The genetic diversity of L. sakei is mainly reflected in carbohydrate utilization, antibiotic tolerance, and immune/competition-related factors, such as clustering regular interval short palindromic repeat sequence (CRISPR)–Cas. The CRISPR system is mainly IIA type, and a few are IIC types. This work provides a basis for the study of this species. MDPI 2021-07-25 /pmc/articles/PMC8393274/ /pubmed/34441495 http://dx.doi.org/10.3390/foods10081717 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Ying
Li, Nan
Zhao, Shenxi
Zhang, Chuan
Qiao, Nanzhen
Duan, Hui
Xiao, Yue
Yan, Bowen
Zhao, Jianxin
Tian, Fengwei
Zhai, Qixiao
Yu, Leilei
Chen, Wei
Integrated Phenotypic–Genotypic Analysis of Latilactobacillus sakei from Different Niches
title Integrated Phenotypic–Genotypic Analysis of Latilactobacillus sakei from Different Niches
title_full Integrated Phenotypic–Genotypic Analysis of Latilactobacillus sakei from Different Niches
title_fullStr Integrated Phenotypic–Genotypic Analysis of Latilactobacillus sakei from Different Niches
title_full_unstemmed Integrated Phenotypic–Genotypic Analysis of Latilactobacillus sakei from Different Niches
title_short Integrated Phenotypic–Genotypic Analysis of Latilactobacillus sakei from Different Niches
title_sort integrated phenotypic–genotypic analysis of latilactobacillus sakei from different niches
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393274/
https://www.ncbi.nlm.nih.gov/pubmed/34441495
http://dx.doi.org/10.3390/foods10081717
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