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Development of an RNA Extraction Protocol for Norovirus from Raw Oysters and Detection by qRT-PCR and Droplet-Digital RT-PCR

Foodborne viruses such as norovirus and hepatitis A virus cause frequent outbreaks associated with the consumption of raw or undercooked oysters. Viral particles are bioaccumulated in the oyster’s digestive glands, making RNA extraction and RT-PCR detection difficult due to the complex nature of the...

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Detalles Bibliográficos
Autores principales: Plante, Daniel, Bran Barrera, Julio Alexander, Lord, Maude, Iugovaz, Irène, Nasheri, Neda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393641/
https://www.ncbi.nlm.nih.gov/pubmed/34441580
http://dx.doi.org/10.3390/foods10081804