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Isolation of High Molecular Weight DNA from the Model Beetle Tribolium for Nanopore Sequencing

The long-read Nanopore sequencing has been recently applied for assembly of complex genomes and analysis of linear genome organization. The most critical factor for successful long-read sequencing is extraction of high molecular weight (HMW) DNA of sufficient purity and quantity. The challenges asso...

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Detalles Bibliográficos
Autores principales: Volarić, Marin, Veseljak, Damira, Mravinac, Brankica, Meštrović, Nevenka, Despot-Slade, Evelin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394269/
https://www.ncbi.nlm.nih.gov/pubmed/34440288
http://dx.doi.org/10.3390/genes12081114