Recombinant protein production provoked accumulation of ATP, fructose-1,6-bisphosphate and pyruvate in E. coli K12 strain TG1

BACKGROUND: Recently it was shown that production of recombinant proteins in E. coli BL21(DE3) using pET based expression vectors leads to metabolic stress comparable to a carbon overfeeding response. Opposite to original expectations generation of energy as well as catabolic provision of precursor...

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Detalles Bibliográficos
Autores principales: Weber, Jan, Li, Zhaopeng, Rinas, Ursula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394631/
https://www.ncbi.nlm.nih.gov/pubmed/34446023
http://dx.doi.org/10.1186/s12934-021-01661-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394631/
https://www.ncbi.nlm.nih.gov/pubmed/34446023
http://dx.doi.org/10.1186/s12934-021-01661-9

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