Preparation and Directed Evolution of Anti-Ciprofloxacin ScFv for Immunoassay in Animal-Derived Food

An immunized mouse phage display scFv library with a capacity of 3.34 × 10(9) CFU/mL was constructed and used for screening of recombinant anti-ciprofloxacin single-chain antibody for the detection of ciprofloxacin (CIP) in animal-derived food. After four rounds of bio-panning, 25 positives were iso...

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Detalles Bibliográficos
Autores principales: Wang, Fangyu, Li, Ning, Zhang, Yunshang, Sun, Xuefeng, Hu, Man, Zhao, Yali, Fan, Jianming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394695/
https://www.ncbi.nlm.nih.gov/pubmed/34441715
http://dx.doi.org/10.3390/foods10081933

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394695/
https://www.ncbi.nlm.nih.gov/pubmed/34441715
http://dx.doi.org/10.3390/foods10081933

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