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Preparation and Directed Evolution of Anti-Ciprofloxacin ScFv for Immunoassay in Animal-Derived Food

An immunized mouse phage display scFv library with a capacity of 3.34 × 10(9) CFU/mL was constructed and used for screening of recombinant anti-ciprofloxacin single-chain antibody for the detection of ciprofloxacin (CIP) in animal-derived food. After four rounds of bio-panning, 25 positives were iso...

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Detalles Bibliográficos
Autores principales:	Wang, Fangyu, Li, Ning, Zhang, Yunshang, Sun, Xuefeng, Hu, Man, Zhao, Yali, Fan, Jianming
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394695/ https://www.ncbi.nlm.nih.gov/pubmed/34441715 http://dx.doi.org/10.3390/foods10081933

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