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A FACS-Free Purification Method to Study Estrogen Signaling, Organoid Formation, and Metabolic Reprogramming in Mammary Epithelial Cells

Few in vitro models are used to study mammary epithelial cells (MECs), and most of these do not express the estrogen receptor α (ERα). Primary MECs can be used to overcome this issue, but methods to purify these cells generally require flow cytometry and fluorescence-activated cell sorting (FACS), w...

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Detalles Bibliográficos
Autores principales: Lacouture, Aurélie, Jobin, Cynthia, Weidmann, Cindy, Berthiaume, Line, Bastien, Dominic, Laverdière, Isabelle, Pelletier, Martin, Audet-Walsh, Étienne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397380/
https://www.ncbi.nlm.nih.gov/pubmed/34456857
http://dx.doi.org/10.3389/fendo.2021.672466