Protocol for CRISPR-Cas9 modification of glycosylation in 3D organotypic skin models

Glycosylation is one of the most common protein modifications in living organisms and has important regulatory roles in animal tissue development and homeostasis. Here, we present a protocol for generation of 3D organotypic skin models using CRISPR-Cas9 genetically engineered human keratinocytes (N/...

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Detalles Bibliográficos
Autores principales: Marinova, Irina N., Wandall, Hans H., Dabelsteen, Sally
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403582/
https://www.ncbi.nlm.nih.gov/pubmed/34485933
http://dx.doi.org/10.1016/j.xpro.2021.100668

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403582/
https://www.ncbi.nlm.nih.gov/pubmed/34485933
http://dx.doi.org/10.1016/j.xpro.2021.100668

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