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Protocol for CRISPR-Cas9 modification of glycosylation in 3D organotypic skin models
Glycosylation is one of the most common protein modifications in living organisms and has important regulatory roles in animal tissue development and homeostasis. Here, we present a protocol for generation of 3D organotypic skin models using CRISPR-Cas9 genetically engineered human keratinocytes (N/...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403582/ https://www.ncbi.nlm.nih.gov/pubmed/34485933 http://dx.doi.org/10.1016/j.xpro.2021.100668 |