EU-RNA-seq for in vivo labeling and high throughput sequencing of nascent transcripts

The protocol allows for labeling nascent RNA without isolating nuclei. The cell-permeable uridine analog, 5-ethynyluridine (EU), is added to media to allow in vivo labeling of nascent transcripts. Cells are lysed, total RNA is collected, and biotin is conjugated to EU-labeled RNAs. Custom biotin RNA...

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Detalles Bibliográficos
Autores principales: Palozola, Katherine C., Donahue, Greg, Zaret, Kenneth S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403648/
https://www.ncbi.nlm.nih.gov/pubmed/34485932
http://dx.doi.org/10.1016/j.xpro.2021.100651

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403648/
https://www.ncbi.nlm.nih.gov/pubmed/34485932
http://dx.doi.org/10.1016/j.xpro.2021.100651

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