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Low-input ATAC&mRNA-seq protocol for simultaneous profiling of chromatin accessibility and gene expression

We present a simple, fast, and robust protocol (low-input ATAC&mRNA-seq) to simultaneously generate ATAC-seq and mRNA-seq libraries from the same cells in limited cell numbers by coupling a simplified ATAC procedure using whole cells with a novel mRNA-seq approach that features a seamless on-bea...

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Detalles Bibliográficos
Autores principales: Li, Ruifang, Grimm, Sara A., Wade, Paul A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403761/
https://www.ncbi.nlm.nih.gov/pubmed/34485936
http://dx.doi.org/10.1016/j.xpro.2021.100764