3D in vitro culture system to study collective migration in mammary organoid epithelium

We recently established an in vitro culture system in which mammary gland organoid undergoes directional migration in response to an FGF10 concentration gradient. Here, we describe a step-by-step protocol for preparing organoids, the setup of the 3D culture system, and the image acquisition approach...

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Detalles Bibliográficos
Autores principales: Lu, Yunzhe, Deng, Ruolan, You, Huanyang, Lu, Pengfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8405933/
https://www.ncbi.nlm.nih.gov/pubmed/34485944
http://dx.doi.org/10.1016/j.xpro.2021.100778

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8405933/
https://www.ncbi.nlm.nih.gov/pubmed/34485944
http://dx.doi.org/10.1016/j.xpro.2021.100778

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