3D in vitro culture system to study collective migration in mammary organoid epithelium

We recently established an in vitro culture system in which mammary gland organoid undergoes directional migration in response to an FGF10 concentration gradient. Here, we describe a step-by-step protocol for preparing organoids, the setup of the 3D culture system, and the image acquisition approach...

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Detalles Bibliográficos
Autores principales: Lu, Yunzhe, Deng, Ruolan, You, Huanyang, Lu, Pengfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8405933/
https://www.ncbi.nlm.nih.gov/pubmed/34485944
http://dx.doi.org/10.1016/j.xpro.2021.100778
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author Lu, Yunzhe
Deng, Ruolan
You, Huanyang
Lu, Pengfei
author_facet Lu, Yunzhe
Deng, Ruolan
You, Huanyang
Lu, Pengfei
author_sort Lu, Yunzhe
collection PubMed
description We recently established an in vitro culture system in which mammary gland organoid undergoes directional migration in response to an FGF10 concentration gradient. Here, we describe a step-by-step protocol for preparing organoids, the setup of the 3D culture system, and the image acquisition approach. The technical difficulties in conducting the 3D migration assay are choosing epithelial organoids of appropriate sizes and manually paring organoids and beads pre-soaked in FGF10 within a desirable distance (∼100 μm). For complete details on the use and execution of this protocol, please refer to Lu et al. (2020).
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spelling pubmed-84059332021-09-02 3D in vitro culture system to study collective migration in mammary organoid epithelium Lu, Yunzhe Deng, Ruolan You, Huanyang Lu, Pengfei STAR Protoc Protocol We recently established an in vitro culture system in which mammary gland organoid undergoes directional migration in response to an FGF10 concentration gradient. Here, we describe a step-by-step protocol for preparing organoids, the setup of the 3D culture system, and the image acquisition approach. The technical difficulties in conducting the 3D migration assay are choosing epithelial organoids of appropriate sizes and manually paring organoids and beads pre-soaked in FGF10 within a desirable distance (∼100 μm). For complete details on the use and execution of this protocol, please refer to Lu et al. (2020). Elsevier 2021-08-27 /pmc/articles/PMC8405933/ /pubmed/34485944 http://dx.doi.org/10.1016/j.xpro.2021.100778 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lu, Yunzhe
Deng, Ruolan
You, Huanyang
Lu, Pengfei
3D in vitro culture system to study collective migration in mammary organoid epithelium
title 3D in vitro culture system to study collective migration in mammary organoid epithelium
title_full 3D in vitro culture system to study collective migration in mammary organoid epithelium
title_fullStr 3D in vitro culture system to study collective migration in mammary organoid epithelium
title_full_unstemmed 3D in vitro culture system to study collective migration in mammary organoid epithelium
title_short 3D in vitro culture system to study collective migration in mammary organoid epithelium
title_sort 3d in vitro culture system to study collective migration in mammary organoid epithelium
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8405933/
https://www.ncbi.nlm.nih.gov/pubmed/34485944
http://dx.doi.org/10.1016/j.xpro.2021.100778
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AT dengruolan 3dinvitroculturesystemtostudycollectivemigrationinmammaryorganoidepithelium
AT youhuanyang 3dinvitroculturesystemtostudycollectivemigrationinmammaryorganoidepithelium
AT lupengfei 3dinvitroculturesystemtostudycollectivemigrationinmammaryorganoidepithelium

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