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3D in vitro culture system to study collective migration in mammary organoid epithelium
We recently established an in vitro culture system in which mammary gland organoid undergoes directional migration in response to an FGF10 concentration gradient. Here, we describe a step-by-step protocol for preparing organoids, the setup of the 3D culture system, and the image acquisition approach...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8405933/ https://www.ncbi.nlm.nih.gov/pubmed/34485944 http://dx.doi.org/10.1016/j.xpro.2021.100778 |