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Glypicans define unique roles for the Hedgehog co-receptors boi and ihog in cytoneme-mediated gradient formation

The conserved family of Hedgehog (Hh) signaling proteins plays a key role in cell–cell communication in development, tissue repair, and cancer progression, inducing distinct concentration-dependent responses in target cells located at short and long distances. One simple mechanism for long distance...

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Autores principales: Simon, Eléanor, Jiménez-Jiménez, Carlos, Seijo-Barandiarán, Irene, Aguilar, Gustavo, Sánchez-Hernández, David, Aguirre-Tamaral, Adrián, González-Méndez, Laura, Ripoll, Pedro, Guerrero, Isabel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410076/
https://www.ncbi.nlm.nih.gov/pubmed/34355694
http://dx.doi.org/10.7554/eLife.64581
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author Simon, Eléanor
Jiménez-Jiménez, Carlos
Seijo-Barandiarán, Irene
Aguilar, Gustavo
Sánchez-Hernández, David
Aguirre-Tamaral, Adrián
González-Méndez, Laura
Ripoll, Pedro
Guerrero, Isabel
author_facet Simon, Eléanor
Jiménez-Jiménez, Carlos
Seijo-Barandiarán, Irene
Aguilar, Gustavo
Sánchez-Hernández, David
Aguirre-Tamaral, Adrián
González-Méndez, Laura
Ripoll, Pedro
Guerrero, Isabel
author_sort Simon, Eléanor
collection PubMed
description The conserved family of Hedgehog (Hh) signaling proteins plays a key role in cell–cell communication in development, tissue repair, and cancer progression, inducing distinct concentration-dependent responses in target cells located at short and long distances. One simple mechanism for long distance dispersal of the lipid modified Hh is the direct contact between cell membranes through filopodia-like structures known as cytonemes. Here we have analyzed in Drosophila the interaction between the glypicans Dally and Dally-like protein, necessary for Hh signaling, and the adhesion molecules and Hh coreceptors Ihog and Boi. We describe that glypicans are required to maintain the levels of Ihog, but not of Boi. We also show that the overexpression of Ihog, but not of Boi, regulates cytoneme dynamics through their interaction with glypicans, the Ihog fibronectin III domains being essential for this interaction. Our data suggest that the regulation of glypicans over Hh signaling is specifically given by their interaction with Ihog in cytonemes. Contrary to previous data, we also show that there is no redundancy of Ihog and Boi functions in Hh gradient formation, being Ihog, but not of Boi, essential for the long-range gradient.
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spelling pubmed-84100762021-09-03 Glypicans define unique roles for the Hedgehog co-receptors boi and ihog in cytoneme-mediated gradient formation Simon, Eléanor Jiménez-Jiménez, Carlos Seijo-Barandiarán, Irene Aguilar, Gustavo Sánchez-Hernández, David Aguirre-Tamaral, Adrián González-Méndez, Laura Ripoll, Pedro Guerrero, Isabel eLife Cell Biology The conserved family of Hedgehog (Hh) signaling proteins plays a key role in cell–cell communication in development, tissue repair, and cancer progression, inducing distinct concentration-dependent responses in target cells located at short and long distances. One simple mechanism for long distance dispersal of the lipid modified Hh is the direct contact between cell membranes through filopodia-like structures known as cytonemes. Here we have analyzed in Drosophila the interaction between the glypicans Dally and Dally-like protein, necessary for Hh signaling, and the adhesion molecules and Hh coreceptors Ihog and Boi. We describe that glypicans are required to maintain the levels of Ihog, but not of Boi. We also show that the overexpression of Ihog, but not of Boi, regulates cytoneme dynamics through their interaction with glypicans, the Ihog fibronectin III domains being essential for this interaction. Our data suggest that the regulation of glypicans over Hh signaling is specifically given by their interaction with Ihog in cytonemes. Contrary to previous data, we also show that there is no redundancy of Ihog and Boi functions in Hh gradient formation, being Ihog, but not of Boi, essential for the long-range gradient. eLife Sciences Publications, Ltd 2021-08-06 /pmc/articles/PMC8410076/ /pubmed/34355694 http://dx.doi.org/10.7554/eLife.64581 Text en © 2021, Simon et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Simon, Eléanor
Jiménez-Jiménez, Carlos
Seijo-Barandiarán, Irene
Aguilar, Gustavo
Sánchez-Hernández, David
Aguirre-Tamaral, Adrián
González-Méndez, Laura
Ripoll, Pedro
Guerrero, Isabel
Glypicans define unique roles for the Hedgehog co-receptors boi and ihog in cytoneme-mediated gradient formation
title Glypicans define unique roles for the Hedgehog co-receptors boi and ihog in cytoneme-mediated gradient formation
title_full Glypicans define unique roles for the Hedgehog co-receptors boi and ihog in cytoneme-mediated gradient formation
title_fullStr Glypicans define unique roles for the Hedgehog co-receptors boi and ihog in cytoneme-mediated gradient formation
title_full_unstemmed Glypicans define unique roles for the Hedgehog co-receptors boi and ihog in cytoneme-mediated gradient formation
title_short Glypicans define unique roles for the Hedgehog co-receptors boi and ihog in cytoneme-mediated gradient formation
title_sort glypicans define unique roles for the hedgehog co-receptors boi and ihog in cytoneme-mediated gradient formation
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410076/
https://www.ncbi.nlm.nih.gov/pubmed/34355694
http://dx.doi.org/10.7554/eLife.64581
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