A versatile genetic engineering toolkit for E. coli based on CRISPR-prime editing

CRISPR base editing is a powerful method to engineer bacterial genomes. However, it restricts editing to single-nucleotide substitutions. Here, to address this challenge, we adapt a CRISPR-Prime Editing-based, DSB-free, versatile, and single-nucleotide resolution genetic manipulation toolkit for pro...

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Detalles Bibliográficos
Autores principales: Tong, Yaojun, Jørgensen, Tue S., Whitford, Christopher M., Weber, Tilmann, Lee, Sang Yup
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410854/
https://www.ncbi.nlm.nih.gov/pubmed/34471126
http://dx.doi.org/10.1038/s41467-021-25541-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410854/
https://www.ncbi.nlm.nih.gov/pubmed/34471126
http://dx.doi.org/10.1038/s41467-021-25541-3

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