Cargando…
Hydroxyl radical mediated damage of proteins in low oxygen solution investigated using X-ray footprinting mass spectrometry
In the method of X-ray footprinting mass spectrometry (XFMS), proteins at micromolar concentration in solution are irradiated with a broadband X-ray source, and the resulting hydroxyl radical modifications are characterized using liquid chromatography mass spectrometry to determine sites of solvent...
Autores principales: | Kristensen, Line G., Holton, James M., Rad, Behzad, Chen, Yan, Petzold, Christopher J., Gupta, Sayan, Ralston, Corie Y. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415330/ https://www.ncbi.nlm.nih.gov/pubmed/34475282 http://dx.doi.org/10.1107/S1600577521004744 |
Ejemplares similares
-
Determination of X-ray flux using silicon pin diodes
por: Owen, Robin L., et al.
Publicado: (2009) -
Structural Investigation of Therapeutic Antibodies Using Hydroxyl Radical Protein Footprinting Methods
por: Ralston, Corie Y., et al.
Publicado: (2022) -
A SAXS-based approach to rationally evaluate radical scavengers – toward eliminating radiation damage in solution and crystallographic studies
por: Stachowski, Timothy R., et al.
Publicado: (2021) -
A beginner’s guide to radiation damage
por: Holton, James M.
Publicado: (2009) -
Low-dose X-ray structure analysis of cytochrome c oxidase utilizing high-energy X-rays
por: Ueno, Go, et al.
Publicado: (2019)