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Microinjection for precision genome editing in Caenorhabditis elegans

In Caenorhabditis elegans, targeted genome editing techniques are now routinely used to generate germline edits. The remarkable ease of C. elegans germline editing is attributed to the syncytial nature of the pachytene ovary which is easily accessed by microinjection. This protocol describes the ste...

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Detalles Bibliográficos
Autores principales: Ghanta, Krishna S., Ishidate, Takao, Mello, Craig C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417391/
https://www.ncbi.nlm.nih.gov/pubmed/34505086
http://dx.doi.org/10.1016/j.xpro.2021.100748