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Microinjection for precision genome editing in Caenorhabditis elegans
In Caenorhabditis elegans, targeted genome editing techniques are now routinely used to generate germline edits. The remarkable ease of C. elegans germline editing is attributed to the syncytial nature of the pachytene ovary which is easily accessed by microinjection. This protocol describes the ste...
Autores principales: | Ghanta, Krishna S., Ishidate, Takao, Mello, Craig C. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417391/ https://www.ncbi.nlm.nih.gov/pubmed/34505086 http://dx.doi.org/10.1016/j.xpro.2021.100748 |
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