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Does chronic dietary exposure to the mycotoxin deoxynivalenol affect the porcine hepatic transcriptome when an acute-phase response is initiated through first or second-pass LPS challenge of the liver?

The sensitivity of pigs to deoxynivalenol (DON) might be increased by systemic inflammation (SI), which also has consequences for hepatic integrity. Liver lesions and a dys-regulated gene network might hamper hepatic handling and elimination of DON whereby the way of initiation of hepatic inflammati...

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Autores principales: Dänicke, Sven, Heymann, Ann-Katrin, Oster, Michael, Wimmers, Klaus, Tesch, Tanja, Bannert, Erik, Bühler, Susanne, Kersten, Susanne, Frahm, Jana, Kluess, Jeannette, Kahlert, Stefan, Rothkötter, Hermann-Josef, Billenkamp, Fabian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419296/
https://www.ncbi.nlm.nih.gov/pubmed/34338001
http://dx.doi.org/10.1177/17534259211030563
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author Dänicke, Sven
Heymann, Ann-Katrin
Oster, Michael
Wimmers, Klaus
Tesch, Tanja
Bannert, Erik
Bühler, Susanne
Kersten, Susanne
Frahm, Jana
Kluess, Jeannette
Kahlert, Stefan
Rothkötter, Hermann-Josef
Billenkamp, Fabian
author_facet Dänicke, Sven
Heymann, Ann-Katrin
Oster, Michael
Wimmers, Klaus
Tesch, Tanja
Bannert, Erik
Bühler, Susanne
Kersten, Susanne
Frahm, Jana
Kluess, Jeannette
Kahlert, Stefan
Rothkötter, Hermann-Josef
Billenkamp, Fabian
author_sort Dänicke, Sven
collection PubMed
description The sensitivity of pigs to deoxynivalenol (DON) might be increased by systemic inflammation (SI), which also has consequences for hepatic integrity. Liver lesions and a dys-regulated gene network might hamper hepatic handling and elimination of DON whereby the way of initiation of hepatic inflammation might play an additional role. First and second-pass exposure of the liver with LPS for triggering a SI was achieved by LPS infusion via pre- or post-hepatic venous route, respectively. Each infusion group was pre-conditioned either with a control diet (0.12 mg DON/kg diet) or with a DON-contaminated diet (4.59 mg DON/kg diet) for 4 wk. Liver transcriptome was evaluated at 195 min after starting infusions. DON exposure alone failed to modulate the mRNA expression significantly. However, pre- and post-hepatic LPS challenges prompted transcriptional responses in immune and metabolic levels. The mRNAs for B-cell lymphoma 2-like protein 11 as a key factor in apoptosis and IFN-γ released by T cells were clearly up-regulated in DON-fed group infused with LPS post-hepatically. On the other hand, mRNAs for nucleotide binding oligomerization domain containing 2, IFN-α and eukaryotic translation initiation factor 2α kinase 3 as ribosomal stress sensors were exclusively up-regulated in control pigs with pre-hepatic LPS infusion. These diverse effects were traced back to differences in TLR4 signalling.
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spelling pubmed-84192962021-09-07 Does chronic dietary exposure to the mycotoxin deoxynivalenol affect the porcine hepatic transcriptome when an acute-phase response is initiated through first or second-pass LPS challenge of the liver? Dänicke, Sven Heymann, Ann-Katrin Oster, Michael Wimmers, Klaus Tesch, Tanja Bannert, Erik Bühler, Susanne Kersten, Susanne Frahm, Jana Kluess, Jeannette Kahlert, Stefan Rothkötter, Hermann-Josef Billenkamp, Fabian Innate Immun Original Articles The sensitivity of pigs to deoxynivalenol (DON) might be increased by systemic inflammation (SI), which also has consequences for hepatic integrity. Liver lesions and a dys-regulated gene network might hamper hepatic handling and elimination of DON whereby the way of initiation of hepatic inflammation might play an additional role. First and second-pass exposure of the liver with LPS for triggering a SI was achieved by LPS infusion via pre- or post-hepatic venous route, respectively. Each infusion group was pre-conditioned either with a control diet (0.12 mg DON/kg diet) or with a DON-contaminated diet (4.59 mg DON/kg diet) for 4 wk. Liver transcriptome was evaluated at 195 min after starting infusions. DON exposure alone failed to modulate the mRNA expression significantly. However, pre- and post-hepatic LPS challenges prompted transcriptional responses in immune and metabolic levels. The mRNAs for B-cell lymphoma 2-like protein 11 as a key factor in apoptosis and IFN-γ released by T cells were clearly up-regulated in DON-fed group infused with LPS post-hepatically. On the other hand, mRNAs for nucleotide binding oligomerization domain containing 2, IFN-α and eukaryotic translation initiation factor 2α kinase 3 as ribosomal stress sensors were exclusively up-regulated in control pigs with pre-hepatic LPS infusion. These diverse effects were traced back to differences in TLR4 signalling. SAGE Publications 2021-07-31 2021-07 /pmc/articles/PMC8419296/ /pubmed/34338001 http://dx.doi.org/10.1177/17534259211030563 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Articles
Dänicke, Sven
Heymann, Ann-Katrin
Oster, Michael
Wimmers, Klaus
Tesch, Tanja
Bannert, Erik
Bühler, Susanne
Kersten, Susanne
Frahm, Jana
Kluess, Jeannette
Kahlert, Stefan
Rothkötter, Hermann-Josef
Billenkamp, Fabian
Does chronic dietary exposure to the mycotoxin deoxynivalenol affect the porcine hepatic transcriptome when an acute-phase response is initiated through first or second-pass LPS challenge of the liver?
title Does chronic dietary exposure to the mycotoxin deoxynivalenol affect the porcine hepatic transcriptome when an acute-phase response is initiated through first or second-pass LPS challenge of the liver?
title_full Does chronic dietary exposure to the mycotoxin deoxynivalenol affect the porcine hepatic transcriptome when an acute-phase response is initiated through first or second-pass LPS challenge of the liver?
title_fullStr Does chronic dietary exposure to the mycotoxin deoxynivalenol affect the porcine hepatic transcriptome when an acute-phase response is initiated through first or second-pass LPS challenge of the liver?
title_full_unstemmed Does chronic dietary exposure to the mycotoxin deoxynivalenol affect the porcine hepatic transcriptome when an acute-phase response is initiated through first or second-pass LPS challenge of the liver?
title_short Does chronic dietary exposure to the mycotoxin deoxynivalenol affect the porcine hepatic transcriptome when an acute-phase response is initiated through first or second-pass LPS challenge of the liver?
title_sort does chronic dietary exposure to the mycotoxin deoxynivalenol affect the porcine hepatic transcriptome when an acute-phase response is initiated through first or second-pass lps challenge of the liver?
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419296/
https://www.ncbi.nlm.nih.gov/pubmed/34338001
http://dx.doi.org/10.1177/17534259211030563
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