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Enzymatic Beacons for Specific Sensing of Dilute Nucleic Acid and Potential Utility for SARS-CoV-2 Detection.
Enzymatic beacons, or E-beacons, are 1:1 bioconjugates of the nanoluciferase enzyme linked covalently at its C-terminus to hairpin forming DNA oligonucleotides equipped with a dark quencher. We prepared E-beacons biocatalytically using the promiscuous “hedgehog” protein-cholesterol ligase, HhC. Inst...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Cold Spring Harbor Laboratory
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8423218/ https://www.ncbi.nlm.nih.gov/pubmed/34494022 http://dx.doi.org/10.1101/2021.08.30.458287 |