Enzymatic Beacons for Specific Sensing of Dilute Nucleic Acid and Potential Utility for SARS-CoV-2 Detection.

Enzymatic beacons, or E-beacons, are 1:1 bioconjugates of the nanoluciferase enzyme linked covalently at its C-terminus to hairpin forming DNA oligonucleotides equipped with a dark quencher. We prepared E-beacons biocatalytically using the promiscuous “hedgehog” protein-cholesterol ligase, HhC. Inst...

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Detalles Bibliográficos
Autores principales: Zhang, Xiaoyu, Kotikam, Venubabu, Rozners, Eriks, Callahan, Brian P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8423218/
https://www.ncbi.nlm.nih.gov/pubmed/34494022
http://dx.doi.org/10.1101/2021.08.30.458287

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8423218/
https://www.ncbi.nlm.nih.gov/pubmed/34494022
http://dx.doi.org/10.1101/2021.08.30.458287

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