A glycosaminoglycan microarray identifies the binding of SARS‐CoV‐2 spike protein to chondroitin sulfate E

Heparan sulfate (HS), a sulfated glycosaminoglycan (GAG), was reported to be a necessary host attachment factor that promotes SARS‐CoV‐2 infection. In this study, we developed GAG microarrays based on fluorescence detection for high‐sensitivity screening of the GAG‐binding specificity of proteins an...

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Autores principales: Watanabe, Tomoko, Takeda, Ko, Hiemori, Keiko, Minamisawa, Toshikazu, Tateno, Hiroaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Research Letters
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8427098/
https://www.ncbi.nlm.nih.gov/pubmed/34375459
http://dx.doi.org/10.1002/1873-3468.14173
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author Watanabe, Tomoko
Takeda, Ko
Hiemori, Keiko
Minamisawa, Toshikazu
Tateno, Hiroaki
author_facet Watanabe, Tomoko
Takeda, Ko
Hiemori, Keiko
Minamisawa, Toshikazu
Tateno, Hiroaki
author_sort Watanabe, Tomoko
collection PubMed
description Heparan sulfate (HS), a sulfated glycosaminoglycan (GAG), was reported to be a necessary host attachment factor that promotes SARS‐CoV‐2 infection. In this study, we developed GAG microarrays based on fluorescence detection for high‐sensitivity screening of the GAG‐binding specificity of proteins and applied it for the analysis of SARS‐CoV‐2 spike (S) protein. Among the 20 distinct GAGs, the S protein bound not only to heparin (HEP)/HS but also to chondroitin sulfate E (CSE) in a concentration‐dependent manner. We then analyzed the specificity of each subunit of the S protein. While the S1 subunit showed exclusive binding to HEP, the S2 subunit also bound to CSE and HEP/HS. CSE might act as an alternative attachment factor for HS in SARS‐CoV‐2 infection.
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spelling pubmed-84270982021-09-09 A glycosaminoglycan microarray identifies the binding of SARS‐CoV‐2 spike protein to chondroitin sulfate E Watanabe, Tomoko Takeda, Ko Hiemori, Keiko Minamisawa, Toshikazu Tateno, Hiroaki FEBS Lett Research Letters Heparan sulfate (HS), a sulfated glycosaminoglycan (GAG), was reported to be a necessary host attachment factor that promotes SARS‐CoV‐2 infection. In this study, we developed GAG microarrays based on fluorescence detection for high‐sensitivity screening of the GAG‐binding specificity of proteins and applied it for the analysis of SARS‐CoV‐2 spike (S) protein. Among the 20 distinct GAGs, the S protein bound not only to heparin (HEP)/HS but also to chondroitin sulfate E (CSE) in a concentration‐dependent manner. We then analyzed the specificity of each subunit of the S protein. While the S1 subunit showed exclusive binding to HEP, the S2 subunit also bound to CSE and HEP/HS. CSE might act as an alternative attachment factor for HS in SARS‐CoV‐2 infection. John Wiley and Sons Inc. 2021-08-17 2021-09 /pmc/articles/PMC8427098/ /pubmed/34375459 http://dx.doi.org/10.1002/1873-3468.14173 Text en © 2021 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Letters
Watanabe, Tomoko
Takeda, Ko
Hiemori, Keiko
Minamisawa, Toshikazu
Tateno, Hiroaki
A glycosaminoglycan microarray identifies the binding of SARS‐CoV‐2 spike protein to chondroitin sulfate E
title A glycosaminoglycan microarray identifies the binding of SARS‐CoV‐2 spike protein to chondroitin sulfate E
title_full A glycosaminoglycan microarray identifies the binding of SARS‐CoV‐2 spike protein to chondroitin sulfate E
title_fullStr A glycosaminoglycan microarray identifies the binding of SARS‐CoV‐2 spike protein to chondroitin sulfate E
title_full_unstemmed A glycosaminoglycan microarray identifies the binding of SARS‐CoV‐2 spike protein to chondroitin sulfate E
title_short A glycosaminoglycan microarray identifies the binding of SARS‐CoV‐2 spike protein to chondroitin sulfate E
title_sort glycosaminoglycan microarray identifies the binding of sars‐cov‐2 spike protein to chondroitin sulfate e
topic Research Letters
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8427098/
https://www.ncbi.nlm.nih.gov/pubmed/34375459
http://dx.doi.org/10.1002/1873-3468.14173
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