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Depletion of PD-1 or PD-L1 did not affect the mortality of mice infected with Mycobacterium avium
The programmed cell death-1 (PD-1) and programmed cell death-ligand 1 (PD-L1) pathway could affect antimicrobial immune responses by suppressing T cell activity. Several recent studies demonstrated that blocking of the PD-1/PD-L1 pathway exacerbated Mycobacterium tuberculosis infection. However, the...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429769/ https://www.ncbi.nlm.nih.gov/pubmed/34504192 http://dx.doi.org/10.1038/s41598-021-97391-4 |
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author | Nakajima, Masayuki Matsuyama, Masashi Kawaguchi, Mio Matsumura, Sosuke Kiwamoto, Takumi Matsuno, Yosuke Morishima, Yuko Yoshida, Kazufumi Sherpa, Mingma Thsering Yazaki, Kai Tanaka, Ryota Okiyama, Naoko Muratani, Masafumi Ishii, Yukio Hizawa, Nobuyuki |
author_facet | Nakajima, Masayuki Matsuyama, Masashi Kawaguchi, Mio Matsumura, Sosuke Kiwamoto, Takumi Matsuno, Yosuke Morishima, Yuko Yoshida, Kazufumi Sherpa, Mingma Thsering Yazaki, Kai Tanaka, Ryota Okiyama, Naoko Muratani, Masafumi Ishii, Yukio Hizawa, Nobuyuki |
author_sort | Nakajima, Masayuki |
collection | PubMed |
description | The programmed cell death-1 (PD-1) and programmed cell death-ligand 1 (PD-L1) pathway could affect antimicrobial immune responses by suppressing T cell activity. Several recent studies demonstrated that blocking of the PD-1/PD-L1 pathway exacerbated Mycobacterium tuberculosis infection. However, the effect of blocking this pathway in pulmonary Mycobacterium avium–intracellulare complex (MAC) infection is not fully understood. Wild-type, PD-1-deficient mice, and PD-L1-deficient mice were intranasally infected with Mycobacterium avium bacteria. Depletion of PD-1 or PD-L1 did not affect mortality and bacterial burden in MAC-infected mice. However, marked infiltration of CD8-positive T lymphocytes was observed in the lungs of PD-1 and PD-L1-deficient mice compared to wild-type mice. Comprehensive transcriptome analysis showed that levels of gene expressions related to Th1 immunity did not differ according to the genotypes. However, genes related to the activity of CD8-positive T cells and related chemokine activity were upregulated in the infected lungs of PD-1 and PD-L1-deficient mice. Thus, the lack of change in susceptibility to MAC infection in PD-1 and PD-L1-deficient mice might be explained by the absence of obvious changes in the Th1 immune response. Furthermore, activated CD8-positive cells in response to MAC infection in these mice seemed to not be relevant in the control of MAC infection. |
format | Online Article Text |
id | pubmed-8429769 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-84297692021-09-13 Depletion of PD-1 or PD-L1 did not affect the mortality of mice infected with Mycobacterium avium Nakajima, Masayuki Matsuyama, Masashi Kawaguchi, Mio Matsumura, Sosuke Kiwamoto, Takumi Matsuno, Yosuke Morishima, Yuko Yoshida, Kazufumi Sherpa, Mingma Thsering Yazaki, Kai Tanaka, Ryota Okiyama, Naoko Muratani, Masafumi Ishii, Yukio Hizawa, Nobuyuki Sci Rep Article The programmed cell death-1 (PD-1) and programmed cell death-ligand 1 (PD-L1) pathway could affect antimicrobial immune responses by suppressing T cell activity. Several recent studies demonstrated that blocking of the PD-1/PD-L1 pathway exacerbated Mycobacterium tuberculosis infection. However, the effect of blocking this pathway in pulmonary Mycobacterium avium–intracellulare complex (MAC) infection is not fully understood. Wild-type, PD-1-deficient mice, and PD-L1-deficient mice were intranasally infected with Mycobacterium avium bacteria. Depletion of PD-1 or PD-L1 did not affect mortality and bacterial burden in MAC-infected mice. However, marked infiltration of CD8-positive T lymphocytes was observed in the lungs of PD-1 and PD-L1-deficient mice compared to wild-type mice. Comprehensive transcriptome analysis showed that levels of gene expressions related to Th1 immunity did not differ according to the genotypes. However, genes related to the activity of CD8-positive T cells and related chemokine activity were upregulated in the infected lungs of PD-1 and PD-L1-deficient mice. Thus, the lack of change in susceptibility to MAC infection in PD-1 and PD-L1-deficient mice might be explained by the absence of obvious changes in the Th1 immune response. Furthermore, activated CD8-positive cells in response to MAC infection in these mice seemed to not be relevant in the control of MAC infection. Nature Publishing Group UK 2021-09-09 /pmc/articles/PMC8429769/ /pubmed/34504192 http://dx.doi.org/10.1038/s41598-021-97391-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Nakajima, Masayuki Matsuyama, Masashi Kawaguchi, Mio Matsumura, Sosuke Kiwamoto, Takumi Matsuno, Yosuke Morishima, Yuko Yoshida, Kazufumi Sherpa, Mingma Thsering Yazaki, Kai Tanaka, Ryota Okiyama, Naoko Muratani, Masafumi Ishii, Yukio Hizawa, Nobuyuki Depletion of PD-1 or PD-L1 did not affect the mortality of mice infected with Mycobacterium avium |
title | Depletion of PD-1 or PD-L1 did not affect the mortality of mice infected with Mycobacterium avium |
title_full | Depletion of PD-1 or PD-L1 did not affect the mortality of mice infected with Mycobacterium avium |
title_fullStr | Depletion of PD-1 or PD-L1 did not affect the mortality of mice infected with Mycobacterium avium |
title_full_unstemmed | Depletion of PD-1 or PD-L1 did not affect the mortality of mice infected with Mycobacterium avium |
title_short | Depletion of PD-1 or PD-L1 did not affect the mortality of mice infected with Mycobacterium avium |
title_sort | depletion of pd-1 or pd-l1 did not affect the mortality of mice infected with mycobacterium avium |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429769/ https://www.ncbi.nlm.nih.gov/pubmed/34504192 http://dx.doi.org/10.1038/s41598-021-97391-4 |
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