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Constructing of Bacillus subtilis-Based Lux-Biosensors with the Use of Stress-Inducible Promoters

Here, we present a new lux-biosensor based on Bacillus subtilis for detecting of DNA-tropic and oxidative stress-causing agents. Hybrid plasmids pNK-DinC, pNK-AlkA, and pNK-MrgA have been constructed, in which the Photorhabdus luminescens reporter genes luxABCDE are transcribed from the stress-induc...

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Detalles Bibliográficos
Autores principales: Kessenikh, Andrew G., Novoyatlova, Uliana S., Bazhenov, Sergey V., Stepanova, Eugeniya A., Khrulnova, Svetlana A., Gnuchikh, Eugeny Yu., Kotova, Vera Yu., Kudryavtseva, Anna A., Bermeshev, Maxim V., Manukhov, Ilya V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431380/
https://www.ncbi.nlm.nih.gov/pubmed/34502476
http://dx.doi.org/10.3390/ijms22179571