Efficient Generation of Knock-In Zebrafish Models for Inherited Disorders Using CRISPR-Cas9 Ribonucleoprotein Complexes

CRISPR-Cas9-based genome-editing is a highly efficient and cost-effective method to generate zebrafish loss-of-function alleles. However, introducing patient-specific variants into the zebrafish genome with CRISPR-Cas9 remains challenging. Targeting options can be limited by the predetermined geneti...

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Detalles Bibliográficos
Autores principales: de Vrieze, Erik, de Bruijn, Suzanne E., Reurink, Janine, Broekman, Sanne, van de Riet, Vince, Aben, Marco, Kremer, Hannie, van Wijk, Erwin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431507/
https://www.ncbi.nlm.nih.gov/pubmed/34502338
http://dx.doi.org/10.3390/ijms22179429

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431507/
https://www.ncbi.nlm.nih.gov/pubmed/34502338
http://dx.doi.org/10.3390/ijms22179429

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