Characterization of Protocatechuate 4,5-Dioxygenase from Pseudarthrobacter phenanthrenivorans Sphe3 and In Situ Reaction Monitoring in the NMR Tube

The current study aims at the functional and kinetic characterization of protocatechuate (PCA) 4,5-dioxygenase (PcaA) from Pseudarthrobacter phenanthrenivorans Sphe3. This is the first single subunit Type II dioxygenase characterized in Actinobacteria. RT-PCR analysis demonstrated that pcaA and the...

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Detalles Bibliográficos
Autores principales: Tsagogiannis, Epameinondas, Vandera, Elpiniki, Primikyri, Alexandra, Asimakoula, Stamatia, Tzakos, Andreas G., Gerothanassis, Ioannis P., Koukkou, Anna-Irini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431788/
https://www.ncbi.nlm.nih.gov/pubmed/34502555
http://dx.doi.org/10.3390/ijms22179647

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431788/
https://www.ncbi.nlm.nih.gov/pubmed/34502555
http://dx.doi.org/10.3390/ijms22179647

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