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Characterization of Protocatechuate 4,5-Dioxygenase from Pseudarthrobacter phenanthrenivorans Sphe3 and In Situ Reaction Monitoring in the NMR Tube
The current study aims at the functional and kinetic characterization of protocatechuate (PCA) 4,5-dioxygenase (PcaA) from Pseudarthrobacter phenanthrenivorans Sphe3. This is the first single subunit Type II dioxygenase characterized in Actinobacteria. RT-PCR analysis demonstrated that pcaA and the...
Autores principales: | Tsagogiannis, Epameinondas, Vandera, Elpiniki, Primikyri, Alexandra, Asimakoula, Stamatia, Tzakos, Andreas G., Gerothanassis, Ioannis P., Koukkou, Anna-Irini |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431788/ https://www.ncbi.nlm.nih.gov/pubmed/34502555 http://dx.doi.org/10.3390/ijms22179647 |
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