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Measuring in vivo protein turnover and exchange in yeast macromolecular assemblies

We present a comprehensive and robust protocol to track the dynamics of all proteins in a complex in yeast cells. A single member of the protein assembly is tagged and conditionally expressed, minimizing the perturbations to the protein complex. Then, SILAC labeling and affinity purification are use...

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Detalles Bibliográficos
Autores principales: Hakhverdyan, Zhanna, Molloy, Kelly R., Subbotin, Roman I., Fernandez-Martinez, Javier, Chait, Brian T., Rout, Michael P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433251/
https://www.ncbi.nlm.nih.gov/pubmed/34527957
http://dx.doi.org/10.1016/j.xpro.2021.100800