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Measuring in vivo protein turnover and exchange in yeast macromolecular assemblies
We present a comprehensive and robust protocol to track the dynamics of all proteins in a complex in yeast cells. A single member of the protein assembly is tagged and conditionally expressed, minimizing the perturbations to the protein complex. Then, SILAC labeling and affinity purification are use...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433251/ https://www.ncbi.nlm.nih.gov/pubmed/34527957 http://dx.doi.org/10.1016/j.xpro.2021.100800 |